The ksms include primarily some fungi and bacteria, but bacteria play the central role in solubilization of k minerals, and widely known as potassium dissolving bacteria kdb or potassium solubilizing bacteria. Potassiumsolubilizing bacteria ksb could serve as inoculants. Ksolubilization is carried out by a large number of saprophytic bacteria bacillus mucilaginosus, b. Ksb are able to solubilize potassium rock through production and secretion of organic acids han and lee, 2005. Potassium is one of the essential nutrients for plant to growth and required in large amount to achieve optimum growth. Influence of potassium solubilizing bacteria on growth and radiocesium accumulation of brassica rapa l. Eliya mursyida, nisa rachmania mubarik and aris tjahjoleksono, 2015. The present study focuses on the phosphate solubilizing capacity of bacteria and fungi in rhizospheres soil samples obtained from. Phosphate solubilizing activities of all isolates were tested on national botanical. Soil potassium supplementation relies heavily on the use of chemical fertilizer, which has a considerable negative impact on the environment. This is a promising strategy for the improvement of plant absorption of potassium and so reducing the use of chemical fertilizer. From the rhizosphere of chinese cabbage plants grown in yangling, we isolated a strain of psb yl6 with a strong ability to. Rhizosphere bacteria have been found to dissolve potassium from insoluble kbearing minerals. These potassium solubilizing bacteria ksb can be used as a promising approach to increase k availability in soils, thus playing an important role for crop establishment under klimited soils.
Five ksb, isolated from banana and chilli rhizosphere were identified by 16s rdna sequence analysis. The biochemical characters are studied for the five isolates of potassium solubilizing bacteria presented in table2. Thus, their application can reduce the consumption of fertilizer and aid in sustainable agricultural development. Nutrient use efficiency and nutrient uptake promoting of rice. Phosphate solubilizing bacteria psb are beneficial bacteria capable of solubilizing inorganic phosphorus from insoluble compounds. Influence of nutritional and environmental conditions priyanka parmar, s. Phosphorus solubilizing bacteria play role in phosphorus nutrition by enhancing its availability to plants through release from inorganic and organic soil p pools by solubilization and mineralization.
Potassiumsolubilizing bacteria and their application in. Percentage of silicate solubilizing bacteria was checked for each soil sample and calculated as follows. Isolation and characterization of potassiumsolubilizing bacteria. Lack of potassium complex in the soil leads to poor development of roots, shoots, less branches, slow grow th, produce smaller seed size with lower yield. They convert insoluble potassium in the soil into a form that plants can access. Potassium solubilization by bacteria ranged from 36. A field experiment was conducted to study the efficacy of indigenous potassium solubilizing bacteria. Occurrence, mechanisms and their role in crop production ahmad ali khan 1, ghulam jilani 1, mohammad saleem akhtar 1, syed muhammad saqlan naqvi 2, mohammad rasheed 3 abstract plants acquire phosphorus from soil solution as phosphate anion. Studies on potassium solubilizing bacteria from southern indian tea soils k.
A small number of studies investigated on the occurrence of phosphate solubilizing bacteria in the marine environment and fresh water environment like lake ecosystem 3, 4. Comparative efficiency of five potash and phosphate. Mikhailouskaya, 2005 isolated potassium solubilizing bacteria from the roots of cereal crop by the use of specific potassium bearing minerals. May 14, 2006 two phosphate and potassium solubilizing strains knp4 and knp414 were isolated from the soil of tianmu mountain, zhejiang province china and they were phenotypically and phylogenetically characterized.
Pdf phosphate and potassium solubilizing bacteria from. Phosphate solubilizing bacteria research papers academia. Pdf isolation and purification of potassium solubilizing. Pdf isolation and characterization of potassium solubilizing. Differences in distribution of potassiumsolubilizing bacteria in. Isolation of phosphate solubilizing bacteria and fungi from. To acquire the optimum outcome by applying this bacterium into the biofertilizer. Isolation and characterization of potassium solubilizing. Beneficial bacteria activate nutrients and promote wheat. Frontiers a complex inoculant of n2fixing, p and k. Potassium solubilizing bacterial strains resulted in release of 35. Isolation and characterization of phosphatesolubilizing. Jun 28, 2016 there are certain microorganisms which use a number of biological processes to make potassium available from unavailable forms. Results showed that rock p and k applied either singly or in combination did not signi.
Isolation, characterization and identification of potassium solubilizing bacteria from rhizosphere soil of maize zea mays. Pdf rock inhabiting potassium solubilizing bacteria from. Microorganisms play a key role in potassium k cycle. Jun 22, 2016 pgp bacteria isolation and determinations of n 2fixing, p and ksolubilizing ability. Potassium solubilizing bacteria ksb solubilize kbearing minerals and change over the insoluble k to dissolvable types of k that plants can get to. Potassium is a vital component of plant nutrition package limiting crop yield and. Pgp bacteria isolation and determinations of n 2fixing, p and k solubilizing ability. Potassium solubilizing microorganisms and their occurrence. Frateuria aurantia was isolated from the plant lilium auratum and from the fruit of the raspberry rubus parvifolius it is a potassium solubilizing bacteria.
Isolation of potassium solubilizing bacteria a total of 37 potassium solubilizing bacteria isolates were obtained from limestone quarry area soil samples of pt indocement tbk, palimanan cirebon using aleksandrov medium. Solubilization of potassium containing various kmineral. The structure and functional activity of the photosynthetic apparatus should be investigated at different levels of its organization. Isolates of ksb was carried out using mica insoluble potassium from the soil sample of different. Frontiers isolation and characterization of a phosphorus. Plant nutrition is closely associated with the activity of plant growth promoting rhizobacteria pgpr, including potassium and zinc solubilizing bacteria ksb and znsb which are used as biofertilizers in many countries where the soils are deficient in available potassium and zinc.
Isolation and purification of potassium solubilizing bacteria. Isolation and characterization of potassiumsolubilizing. Rock inhabiting potassium solubilizing bacteria from kerala, india. An overview krishnananda pralhad ingle1 and dipika ashokrao padole2 biotechnology centre, dr. The integrated use of rock phosphate and psm add 2030 kg p2o5ha.
Biofertilizer is a good platform to deliver this primary macronutrient by assistance of potassium solubilizing bacteria. Effect of coinoculation with phosphate and potassium. This study aimed to evaluate the capacity of pgpb, identified by phenotypic and 16s rrna sequencing from a vegetable purple soil in chongqing, china, to increase soil nitrogen n, phosphorus p, and potassium k availability and growth of. Selection and identification of phosphatepotassium. Our findings show that integration of p and k rocks with inoculation of p and ksolubilising bacteria. Phosphate solubilizing bacteria psb can promote the dissolution of insoluble phosphorus p in soil, enhancing the availability of soluble p. Sharavanan2 1bharathiar university, coimbatore 641 046, tamil nadu, india 2annamalai university, annamalai nagar 608 002, tamil nadu, india corresponding author a b s t r a c t. Badr41 studied potassium and phosphorus solubilization capacity of silicate solubilizing bacteria and it ranged from 490 mg to 758 mg l1 at ph 6. Molecular characterization of potassium solubilizing. Phosphate solubilizing bacteria psb play an important role in biogeochemical phosphorus cycling in both terrestrial and aquatic environments. Phosphate solubilization all of the isolated bacterial isolates were checked for phosphate solubilization on pikoviskaya agar plates 1618. Jun 22, 2016 limited information is available if plant growth promoting bacteria pgpb can promote the growth of fruit crops through improvements in soil fertility. Phosphate solubilizing biofertilizers as a component of inm many heterotropjic bacteria and fungi efficiently solubilize insoluble phosphate of soil and rock phosphates.
Phosphate solubilizing bacteria and fungi play an important role in p bioavailability and can be used to increase pue bolan et al. Many bacteria such as acidothiobacillus ferrooxidans, paenibacillus spp. Ahmad m, nadeem sm, naveed m, zahir za 2016 potassium solubilizing bacteria and their application in agriculture. Potassium solubilizing bacteria will grow on this medium and form. The isolates has shown the solubilization zone ranging 2188 screening of zinc solubilizing and potassium releasing bacterial and fungal isolates from a maximum of 58 to a minimum of 8 mm was found after efficiency was recorded by ksb1 with the. Phosphate and potassium solubilizing bacteria from weathered materials of denatured rock mountain, ha tien, kien giang province, vietnam. The bacteria and fungi convert nonlabile p through either inorganic p solubilizing or organic p mineralization processes. Isolation and identification of potassiumsolubilizing. Isolation and screening of silicate bacteria from various. Inoculation of phosphate solubilizing micro organisms increase crop yield by 510 %.
Muentz showed the first evidence of microbial involvement in solubilization of rock potassium. Isolation and characterization of potassium solubilizing bacteria in. Most of these isolates are aerobic, capsulated, motile and 2 of them are gram positive while 3 are gram negative. Different salts provides various essential ions required for promoting growth of zinc. Isolation and identification of potential phosphate.
Characterization and possibility in chemical k fertilizer substitution article pdf available in journal of basic microbiology. Of phosphate solubilizing bacteria and their coinoculation efficiency on tomato. A significant increase in k solubilization on mica containing broth was achieved by inoculation of different isolates of potassium solubilizing bacteria ksb. The potassium solubilizing bacteria isolates were characterized by a clear zone that formed around the colony figure 1.
Both isolates effectively dissolved mineral phosphate and potassium, while strain knp414 showed higher dissolution capacity even than bacillus mucilaginosus as1. Isolation and characterization of phosphate solubilizing. Potassium solubilizing bacteria ksb such as bacillus mucilagenosus and bacillus edaphicus are example of microorganisms that used in biofertilizer. The bacteria could dissolve nonreadily available forms of soil phosphorus and also potassium and play an essential role in. The utilization of potassiumsolubilizing bacteria ksb to increase the soluble k content in soil has been regarded as a desirable pathway to. Symbionk is a biofertilizer based on a selective strain of potash solubilizing, beneficial bacteria frateuria aurantia. The results indicated that the bacteria we isolated dissolve insoluble phosphatepotassium mainly by producing organic acids. Pdf potassium solubilizing bacteria ksb researchgate. Comparative efficiency of five potash and phosphate solubilizing bacteria and their key enzymes useful for enhancing and improvement of soil fertility sajidmohammad a saiyad, dr. After 5 days, serial dilution of the enriched medium was carried out and.
Tofazzal islama,b, abhinandan deoraa, yasuyuki hashidokoa, atiqur rahmana, toshiaki itoa, and satoshi taharaa a graduate school of agriculture, hokkaido university, kitaku, sapporo 0608589, japan. Selection and identification of phosphate potassium solubilizing bacteria from the area around the limestone mining in cirebon quarry. Isolation, screening and characterization of phosphate. Aleksandrow agar himedia leading biosciences company. An attempt was made to mobilize potassium k from a kbearing mineral waste mica inoculated with k solubilizing bacteria bacillus mucilaginosus as a function of temperature and incubation period under in vitro laboratory conditions. Zinc solubilising bacteria are potential alternates for zinc supplement. Phosphate solubilizing bacteria pdf phosphate solubilizing bacteria pdf download. International journal of current microbiology and applied. Potassium is vital component of plant nutrition package limiting crop yield and quality that performs a multitude of important biological functions to maintain plant growth, isolation of potassium solubilizers was carried out using feldspar. Effect of potassium and phosphorus solubilizing capsicum. Potassium solubilizing bacteria ksb solubilize kbearing minerals and change over the insoluble k to dissolvable types of k that plants can.
Stanford libraries official online search tool for books, media, journals, databases, government documents and more. The concentrations of soluble potassium in the soil are usually very low and more than 90% of potassium in the soil exists in the form of insoluble rocks and silicate minerals. Zinc solubilizing bacteria solubilize both the insoluble zinc compounds, though zno is more effectively solubilized in comparison to znco3 1. Perspectives of potassium solubilizing microbes in. Two phosphate and potassiumsolubilizing bacteria isolated. Potential bacteria such as phosphate solubilizing bacteria and potassium solubilizing bacteria are successfully found from rhizospheric soils of apple from district baramula, jammu and kashmir, india. Rallos agriculture research section philippine nuclear research institute technical workshop on remediation of radioactive contamination in agriculture.
Potassium k is the third major essential macronutrient for plant growth. The biochemical characters are studied for the five isolates of potassium solubilizing bacteria presented in. Phosphate solubilizing bacteria an overview sciencedirect. The ability of a few soil bacteria to transform unavailable forms of potassium k to an available form is an important feature in plant. In this study, isolation, screening and characterization of 35 isolates of phosphate solubilizing bacteria from different regions of karwar were carried out. Feb 21, 2020 potassiumsolubilizing bacteria can also dissolve tricalcium phosphate in medium tables tables1 1 and and2 2 and additional file 3. Salts present in the medium support the growth of potassium solubililizing bacteria by providing the essential nutrients.
Prajapati and others published isolation and characterization of potassium solubilizing bacteria from ceramic industry soil find, read. Biofertilizer is a good platform to deliver this primary macronutrient by assistance of potassium solubilizing bacteria ksb. The morphological and the biochemical characteristics of the 5 good potassium solubilizers are presented in tables 2. Fundamentally, potassium is a macronutrient in plants. Isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of oryza sativa l. A complex inoculant of n2fixing, p and ksolubilizing. Effect of k solubilizing bacterial isolates on ph, ec soluble k and residual silica at 7, 14, 21 and 28 days after incubation in broth containing mica was studied. Potassium solublisers international journal of current.
Potassium solubilizing bacteria will grow on this medium and form a clear zone around the colony, formed due to potassium solubilization in the vicinity of the. Phosphate solubilizing bacteria from sunflower rhizosphere 439 centrifuged at 10,000 rpm for 15 minutes. Sindhu department of microbiology, ccs haryana agricultural university, hisar, 125004, india. The source of potassium salts is potassium alumino silicates. Psolubilization ability of rhizosphere microorganisms is considered to be one of the most important traits associated with plant phosphate nutrition. View phosphate solubilizing bacteria research papers on academia. Potassium solubilizing bacteria ksb could serve as inoculants.
F eldspar and mica are the common potassium supplement for soil. It is known that potassium solubilizing bacteria ksb can solubilize kbearing minerals and convert the insoluble. The potassium solubilizing microorganisms ksms are a rhizospheric microorganism which solubilizes the insoluble potassium k to soluble forms of k for plant growth and yield. Studies on potassium solubilizing bacteria from southern indian. These two efficient k solubilizing bacteria and one phosphorus solubilizing bacteria were examined for their performance on maize. Suspension was spread on pikovskaya agar composition of 1 l medium. It is known that potassium solubilizing bacteria ksb can solubilize kbearing minerals and convert the insoluble k to soluble forms of k available to plant uptake. Potassium is one of the important component of plant nutrients to meet the requirement of plants growth, crop yield and quality of product. Symbionk, biofertilizer, potash solubilizing, bacteria. Studies on dual inoculation of potassium solubilizing bacteria and phosphorus solubilizing bacteria on growth and yield of maize zea mays l.
In potassium solubilizing microorganisms for sustainable agriculture pp. These microbial isolates showed potassium solubilization index from 1. Zaidi a, khan m, ahemad m, oves m 2009 plant growth promotion by phosphate solubilizing bacteria. The cultures were incubated at room temperature for 4872 hours.
Potassium solubilizing microorganisms for sustainable. Application of chemical fertilizers has a considerably negative impact on environmental sustainability. Isolation of phosphate solubilizing bacteria and their ability to solubilize calcium phosphate from the rhizosphere soils tomato, jasmine, onion, and maize, the total of 12 bacterial species of 32 samples were found enriched on pikovskayas agar plates. Therefore potassium solubilizing bacteria are extensively used as biofertilizers. This procedure was repeated five times and the obtained suspensions from each plot were mixed and then diluted with. The pdfloadimage example use of phosphate solubilizing bacteria as inoculants. Rock phosphatepotassium and rocksolubilising bacteria as. Effects of bioleaching on the chemical, mineralogical and morphological properties of natural and wastederived alkaline materials. Phosphate solubilizing bacteria psb plays an important role in soil by. Owing to naturally available source of potassium in soil and high price of synthetic potassium fertilizers, the importance of ksb is increasing day by day.
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